The book has been divided into chapters which will guide the readers for better understanding for different aspects starting from isolation to usage and safety of probiotics. It also includes the mechanisms of action, animal, and human studies as a support for these properties. The book covers the various probiotic products available in the market and discusses the functional roles of probiotics in human health for the well-being by analyzing different intestinal disorders. Highlights the ability of probiotics to modulate gut microbiota and dietary management. It discusses various advancements made towards the preparation of probiotic food in the market. In totality, the book gives an overview of the fundamental concepts, mechanisms, therapeutic actions, technological aspects, and future research related with probiotic bacteria. The book will be helpful for students, scientists, pharmacist’s, nutritionists, scientists working in the field of gastrointestinal disorders and other diseases, companies which are into new functional foods or nutraceuticals. It will also be helpful to the professionals in public health and to clinicians. The book has brought about the successful link between food and health.