As a fundamental concept, gel electrophoresis is a biotechnology method in which macromolecules such as DNA, RNA or protein are fragmented according to their physical characteristics such as molecular mass or charge. These molecules are enforced throughout a porous gel matrix in the influence of electric field enabling countless functions and utilizations. This book is not all-inclusive but still covers a majority of applications of this technique in the varied fields of medical and life sciences. This book is divided into four sections: Electrophoresis Application in Enzymology, Temporal Temperature Gel Electrophoresis, Two-Dimensional Gel Electrophoresis (2-DE) and Other Applications of Gel Electrophoresis Technique. We have made an attempt to keep the data of the book detailed and wide-ranging, and we hope that it will benefit the readers.